Facts About attune flow cytometer Revealed
Facts About attune flow cytometer Revealed
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Information-enriched characterization of mobile populations. Morphological data from visuals can insert into the richness of flow cytometry data. Such as, the determine shows an normally typical apoptosis assay employing Annexin V and PI, including mobile imaging to characterize cells in Just about every inhabitants to reveal morphologically distinctive features. These insights could not are acquired from multiplex staining by yourself.
Regardless of whether you’re a novice or a professional, explore our assets to advance your investigate and expertise in flow cytometry.
Incorporate acoustic focusing technological innovation with substantial-parameter analysis for versatile spectral unmixing and traditional workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing by using a large-speed brightfield camera for simultaneous superior throughput flow cytometry and superior resolution brightfield imaging.
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Moreover, it is actually illuminating to see the number of doublets slip through standard singlet gates, and great to confirm the status of unpredicted double-positive situations in my analysis."
Optimizing gating strategy. Even strong handbook singlet gating is error-susceptible and stays a subjective conclusion stage in Practically all flow cytometry assays. Imaging may be used to substantiate and modify gates to include only single cells of fascination.
Our scientists will structure panels to suit your needs as again and again you would like—cost-free, no item acquire desired.
The Attune NxT cytometer would be the responsible workhorse instrument your lab can trust in for consistent and trustworthy efficiency. The Attune CytPix product contains a large-speed brightfield digital camera and AI-driven picture Examination software, with the latest updates which includes machine learning instruments and an intuitive user interface, enabling enhanced info precision, precision, and speedier insights into sample biology.
Possibly most of all, these activities include cells of Evidently various phenotypes which could lead on to incorrect conclusions about double positive functions (specifically in exceptional populations).
Sound waves permit exact cell alignment, causing Extraordinary facts excellent and ~10X quicker sample flow rates.
We Beforehand shown the power of imaging Vehicle-T/Ramos cell interactions. Permit’s evaluate just the populace of finest desire, the double positive functions, To find out more. We can now use extended morphological parameters (listed here, circularity and skewness of intensity) to even further take a look at the options of these populations and refine gating on these activities, raising information robustness.
Created-in dimensionality reduction equipment elevated resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to substantiate anticipated population resolution in lysed whole human blood. Person can look at functions from Each and every cluster for visual affirmation of mobile more info phenotypes.
Flexible payment strategy from Agilent Monetary Remedies enable you to get the essential instrumentation you require for fast challenge, or to increase your lab.
At the heart of every Attune Flow Cytometer lies our innovative acoustic concentrating technological know-how, which makes use of seem waves to meticulously align cells within the flow mobile. This ground breaking tactic surpasses classic hydrodynamic strategies, resulting in quicker processing speeds, Extraordinary information excellent, and improved sample throughput.
The Attune Xenith cytometer is often a large-parameter spectral analyzer, giving the two spectral unmixing and standard flow abilities for extensive and functional mobile Investigation.
The Attune CytPix Flow Cytometer will allow buyers to visualise samples all through acquisition, verify the precision of gating strategies, and assistance benefits with responsible documentation for publication purposes.
What gave the impression to be a homogeneous populace is really far more varied—an insight that ought to be regarded when interpreting outcomes.